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ASTM E2563 - 18 - Standard Practice for Enumeration of Non-Tuberculosis Mycobacteria in Aqueous Metalworking Fluids by Plate Count Method
This practice allows for the recovery and enumeration of viable and culturable, non-tuberculosis, rapidly growing Mycobacteria ( M. immunogenum , M. chelonae , M. absessus , M. fortuitum , and M. smegmatis ) in the presence of high Gram-negative background populations in metalworking fluid field samples. During the past decade, it has become increasingly apparent that non-tuberculous Mycobacteria are common members of the indigenous MWF bacterial population. This population is predominantly comprised of Gram-negative bacteria and fungi. Mycobacterial contamination of metalworking fluids has been...
ASTM E2564 - 18 - Standard Practice for Enumeration of Mycobacteria in Metalworking Fluids by Direct Microscopic Counting (DMC) Method
During the past decade, it has become increasingly apparent that non-tuberculous mycobacteria are common members of the indigenous MWF bacterial population. Measurement of mycobacterial cell count densities is an important step in establishing a possible relationship between mycobacteria and occupational health-related allergic responses, for example, hypersensitivity pneumonitis (HP) in persons exposed to aerosols of metalworking fluids. It is known that the viable mycobacteria count underestimates the total mycobacterial levels by not counting the non-culturable, possibly dead or moribund population...
Développement d’une approche par séquençage de nouvelle génération pour l’étude de la diversité fongique des bioaérosols
Afin d'explorer l'association entre les bioaérosols, l'exposition aux moisissures et les effets sur la santé respiratoire, l'objectif général de cette activité de recherche était de proposer une méthode d'analyse permettant de déterminer, de manière quantitative, la diversité fongique des bioaérosols, sans égard à leur culture. D'une manière plus spécifique, deux marqueurs génomiques fongiques ont été ciblés par l'approche de séquençage...
Évaluation de la biomasse mycologique sur les surfaces des réseaux aérauliques des systèmes de ventilation
Les systèmes de chauffage, de ventilation et de climatisation de l'air (CVCA) permettent le maintien d'une bonne qualité de l'air dans différents types d'édifices. Toutefois, lorsque ces systèmes ne sont pas bien entretenus, ils peuvent devenir des sources d'émission de polluants dans l'air ambiant. Actuellement, les critères de déclenchement du nettoyage des conduits de ventilation sont souvent basés sur les dépôts de poussières. Or, une évaluation de la biomasse mycologique des poussières...
Comparison of methods to evaluate the fungal biomass in heating, ventilation, and air-conditioning (HVAC) dust
Heating, ventilation, and air-conditioning (HVAC) systems contain dust that can be contaminated with fungal spores (molds), which may have harmful effects on the respiratory health of the occupants of a building. HVAC cleaning is often based on visual inspection of the quantity of dust, without taking the mold content into account. The purpose of this study is to propose a method to estimate fungal contamination of dust in HVAC systems. Comparisons of different analytical methods were carried out on dust deposited in a controlled-atmosphere exposure chamber. Sixty samples were analyzed using four...
Waste Workers’ Exposure to Airborne Fungal and Bacterial Species in the Truck Cab and During Waste Collection
A large number of people work with garbage collection, and exposure to microorganisms is considered an occupational health problem. However, knowledge on microbial exposure at species level is limited. The aim of the study was to achieve knowledge on waste collectors' exposure to airborne inhalable fungal and bacterial species during waste collection with focus on the transport of airborne microorganisms into the truck cab. Airborne microorganisms were collected with samplers mounted in the truck cab, on the workers' clothes, and outdoors. Fungal and bacterial species were quantified and...

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